Glass blocks both the player and enemies from moving through it but it does not block enemy line of sight and projectiles. If glass is hit by a projectile or a melee attack, it will break, broken glass behaves the same way as unbroken glass, except it will allow flames from a flamethrower to travel through. Glass is often used in levels to create areas that enemies can fire at the player from without the player being able to go too close to them.
- Levels with a large amount of glass, such as Dead Ahead are often considered to be some of the harder levels in the Hotline Miami series.
- Although unbroken glass normally will stop flames from a flamethrower from passing through, a glitch exists that allows the player to shoot through windows with a flamethrower, this can be done by walking right up to the windows and firing.